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Genetic vectors --- Molecular cloning --- Vecteurs de clonage --- Clonage moléculaire --- Genetic vectors. --- Molecular cloning.

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This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Biological techniques --- Biochemical engineering --- Molecular biology --- Molecular cloning --- Recombinant DNA --- Laboratory manuals. --- Cloning, Molecular --- DNA, Recombinant --- Clonage moléculaire --- laboratory manuals. --- Laboratory manuals --- Manuels de laboratoire --- Molecular cloning - Laboratory manuals. --- Recombinant DNA - Laboratory manuals.

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Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones.

Biological techniques --- Enzymology --- Molecular biology --- Molecular cloning --- Clonage moléculaire --- Cloning, Molecular --- methods --- molecular cloning --- Expression des gènes --- gene expression --- Expérimentation en laboratoire --- Laboratory experimentation --- methods. --- Methods --- Methods. --- Clonage moléculaire --- Cloning, Molecular - methods --- NUCLEIC ACIDS --- DNA, RECOMBINANT --- MOLECULAR CLONING --- LABORATORY MANUALS --- TECHNIQUES --- Sequencing

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Biomedical Technology --- Organismes génétiquement modifiés --- Emploi en thérapeutique --- Organismes génétiquement modifiés --- Emploi en thérapeutique --- Biotechnology. --- Organisms, Genetically Modified. --- Computational Biology --- Bioengineering --- Genetic engineering --- Transgenic organisms --- Molecular cloning --- Biomolecules --- Bioinformatics --- Medical innovations --- Pharmacology --- Biotechnologie --- Génie génétique --- Clonage moléculaire --- Biomolécules --- Bio-informatique --- Médecine --- Pharmacologie --- Therapeutic use --- Technological innovations --- Innovations --- Computational Biology. --- Biomedical Technology. --- Biotechnologie.

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Basic sciences. molecular biology --- Cloning, molecular. --- Dna, recombinant. --- Genetic engineering. --- Molecular genetics. --- Genetic Engineering. --- Cloning, Molecular. --- DNA, Recombinant. --- Gentechnologie. --- Molecular cloning. --- DNA. --- Clonage moléculaire --- ADN --- Génie génétique --- Genetica --- PCR --- 575.08:577.21 --- 575.21 --- 575.21 Phenotypic variation. Phenotype --- Phenotypic variation. Phenotype --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Genetic engineering, genetic manipulation, recombinant DNA --- Genetic Engineering --- DNA, Recombinant